unable to read ...RealignParameter\Sub_001\_Sub_001.txt

Submitted by xiangrui on

Dear developers and users,

We ran into following error with DPARSFA.

I tried single/two subjects, single/two sessions, and always had problem to read file:
 
The error says the code unable to read file ...\RealignParameter\Sub_3047\_Sub_3047.txt
which happens at Line 2058 of DPARSFA_run.m
 
The file doesn't exist indeed. It seems to me the file name is not constructed correctly, implying by the starting underscore for the name. 
 
Attached is the the auto save file, in case it is useful for diagnoses.
 
Also we had a separate problem to use multi-workers. We can start 4 workers from either command line or DPARSFA GUI without problem, showing by the icon at bottom-right of command window. However, after "Run" DPARSFA, it always error out, and the worker icon shows 0 workers.
 
Does anyone have idea how we can fix the problem? Thanks.
 
Xiangrui

YAN Chao-Gan

Sun, 09/29/2013 - 03:49

Hi Xiangrui,

From the DPARSFA .mat file, seems there is no clue of this error.

Could you paste the full log in the command window here?

Best,

Chao-Gan

Thanks for your quick reply, Chao-Gan.

Below is the full command window output (really long).  

Thank you for the toolbox, and thank you for your time to help us.

-Xiangrui

 

=====================================================

>> DPARSFA
Welcome: li.2327, 2013-09-29 10:13 
Data Processing Assistant for Resting-State fMRI (DPARSF) Advanced Edition (alias: DPARSFA). 
Release=V2.3_130615
Copyright(c) 2009; GNU GENERAL PUBLIC LICENSE
The Nathan Kline Institute for Psychiatric Research, 140 Old Orangeburg Road, Orangeburg, NY 10962; Child Mind Institute, 445 Park Avenue, New York, NY 10022; The Phyllis Green and Randolph Cowen Institute for Pediatric Neuroscience, New York University Child Study Center, New York, NY 10016
State Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, China
Mail to Author:  YAN Chao-Gan
http://www.restfmri.net
-----------------------------------------------------------
Citing Information:
If you think DPARSFA is useful for your work, citing it in your paper would be greatly appreciated.
Something like "... The preprocessing was carried out by using Data Processing Assistant for Resting-State fMRI (DPARSF) (Yan & Zang, 2010, http://www.restfmri.net) which is based on Statistical Parametric Mapping (SPM8) (http://www.fil.ion.ucl.ac.uk/spm) and Resting-State fMRI Data Analysis Toolkit (REST, Song et al., 2011. http://www.restfmri.net)..."
Reference: Yan C and Zang Y (2010) DPARSF: a MATLAB toolbox for "pipeline" data analysis of resting-state fMRI. Front. Syst. Neurosci. 4:13. doi:10.3389/fnsys.2010.00013;     Song, X.W., Dong, Z.Y., Long, X.Y., Li, S.F., Zuo, X.N., Zhu, C.Z., He, Y., Yan, C.G., Zang, Y.F., 2011. REST: A Toolkit for Resting-State Functional Magnetic Resonance Imaging Data Processing. PLoS ONE 6, e25031.
Warning: File: DPARSFA_run.m Line: 658 Column: 60
The temporary variable SliceOrder will be cleared at the beginning of each iteration of the parfor loop.
Any value assigned to it before the loop will be lost.  If SliceOrder is used before it is assigned in the
parfor loop, a runtime error will occur.
See Parallel for Loops in MATLAB, "Temporary Variables". 
> In DPARSFA>pushbuttonRun_Callback at 1601
  In gui_mainfcn at 96
  In DPARSFA at 33 
Warning: File: DPARSFA_run.m Line: 930 Column: 83
The temporary variable RefFile will be cleared at the beginning of each iteration of the parfor loop.
Any value assigned to it before the loop will be lost.  If RefFile is used before it is assigned in the
parfor loop, a runtime error will occur.
See Parallel for Loops in MATLAB, "Temporary Variables". 
> In DPARSFA>pushbuttonRun_Callback at 1601
  In gui_mainfcn at 96
  In DPARSFA at 33 
Slice Timing Setup:Sub_3047 OK
Warning: Run spm_jobman('initcfg'); beforehand 
> In spm_jobman at 107
  In DPARSFA_run>(parfor body) at 685
  In parallel_function at 483
  In DPARSFA_run at 581
  In DPARSFA>pushbuttonRun_Callback at 1601
  In gui_mainfcn at 96
  In DPARSFA at 33 
 
 
------------------------------------------------------------------------
Running job #1
------------------------------------------------------------------------
Running 'Slice Timing'
 
SPM8: spm_slice_timing (v3756)                     10:14:08 - 29/09/2013
========================================================================
Your TR is 2.5
Done    'Slice Timing'
Done
 
Warning: Directory already exists. 
> In DPARSFA_run>(parfor body) at 695
  In parallel_function at 465
  In DPARSFA_run at 694
  In DPARSFA>pushbuttonRun_Callback at 1601
  In gui_mainfcn at 96
  In DPARSFA at 33 
Moving Slice Timing Corrected Files:Sub_3047 OK
Realign Setup:Sub_3047 OK
 
 
------------------------------------------------------------------------
Running job #1
------------------------------------------------------------------------
Running 'Realign: Estimate & Reslice'
Done    'Realign: Estimate & Reslice'
Done
 
Warning: Directory already exists. 
> In DPARSFA_run at 765
  In DPARSFA>pushbuttonRun_Callback at 1601
  In gui_mainfcn at 96
  In DPARSFA at 33 
Warning: Directory already exists. 
> In DPARSFA_run>(parfor body) at 771
  In parallel_function at 465
  In DPARSFA_run at 769
  In DPARSFA>pushbuttonRun_Callback at 1601
  In gui_mainfcn at 96
  In DPARSFA at 33 
Warning: Directory already exists. 
> In DPARSFA_run>(parfor body) at 787
  In parallel_function at 465
  In DPARSFA_run at 785
  In DPARSFA>pushbuttonRun_Callback at 1601
  In gui_mainfcn at 96
  In DPARSFA at 33 
Moving Head Motion Corrected Files:Sub_3047 OK
Warning: Directory already exists. 
> In DPARSFA_run>(parfor body) at 1245
  In parallel_function at 465
  In DPARSFA_run at 1243
  In DPARSFA>pushbuttonRun_Callback at 1601
  In gui_mainfcn at 96
  In DPARSFA at 33 
Copying T1 image Files:Sub_3047 OK
Coregister Setup:Sub_3047 OK
 
 
------------------------------------------------------------------------
Running job #1
------------------------------------------------------------------------
Running 'Coregister: Estimate'
iteration 1...
0.9368   0        0        0        0        0        | -1.0695
0.9368   0.5735   0        0        0        0        | -1.0698
0.9368   0.5735   -2.213   0        0        0        | -1.0717
0.9368   0.5735   -2.213   -0.0353  0        0        | -1.0726
0.9368   0.5735   -2.213   -0.0353  0.01403  0        | -1.0729
0.9368   0.5735   -2.213   -0.0353  0.01403  0.003249 | -1.0729
iteration 2...
0.4082   0.5735   -2.213   -0.0353  0.01403  0.003249 | -1.0733
0.4082   1.113    -2.213   -0.0353  0.01403  0.003249 | -1.0735
0.4082   1.113    -2.456   -0.0353  0.01403  0.003249 | -1.0735
0.4082   1.113    -2.456   -0.044   0.01403  0.003249 | -1.0736
0.4082   1.113    -2.456   -0.044   0.02341  0.003249 | -1.0738
0.4082   1.113    -2.456   -0.044   0.02341  0.006159 | -1.0738
-0.01413 1.544    -2.65    -0.05096 0.03091  0.008484 | -1.074
iteration 3...
-0.01413 1.544    -2.65    -0.05096 0.03091  0.008484 | -1.074
-0.01413 1.536    -2.65    -0.05096 0.03091  0.008484 | -1.074
-0.01413 1.536    -2.31    -0.05096 0.03091  0.008484 | -1.0741
-0.01413 1.536    -2.31    -0.04921 0.03091  0.008484 | -1.0741
-0.01413 1.536    -2.31    -0.04921 0.03254  0.008484 | -1.0741
-0.01413 1.536    -2.31    -0.04921 0.03254  0.009834 | -1.0741
iteration 4...
-0.1703  1.696    -2.381   -0.05178 0.03532  0.01069  | -1.0741
-0.1703  1.54     -2.381   -0.05178 0.03532  0.01069  | -1.0742
-0.1703  1.54     -2.178   -0.05178 0.03532  0.01069  | -1.0742
-0.1703  1.54     -2.178   -0.04959 0.03532  0.01069  | -1.0742
-0.1703  1.54     -2.178   -0.04959 0.0382   0.01069  | -1.0742
-0.1703  1.54     -2.178   -0.04959 0.0382   0.007607 | -1.0742
-0.2553  1.542    -2.106   -0.0498  0.04128  0.006396 | -1.0742
iteration 5...
-0.2553  1.542    -2.106   -0.0498  0.04128  0.006396 | -1.0742
-0.2553  1.418    -2.106   -0.0498  0.04128  0.006396 | -1.0743
-0.2553  1.418    -2.144   -0.0498  0.04128  0.006396 | -1.0743
-0.2553  1.418    -2.144   -0.0492  0.04128  0.006396 | -1.0743
-0.2553  1.418    -2.144   -0.0492  0.04079  0.006396 | -1.0743
-0.2553  1.418    -2.144   -0.0492  0.04079  0.00744  | -1.0743
iteration 6...
-0.299   1.419    -2.107   -0.04931 0.04238  0.006815 | -1.0743
-0.299   1.371    -2.107   -0.04931 0.04238  0.006815 | -1.0743
-0.299   1.371    -2.1     -0.04931 0.04238  0.006815 | -1.0743
-0.299   1.371    -2.1     -0.04896 0.04238  0.006815 | -1.0743
-0.299   1.371    -2.1     -0.04896 0.04151  0.006815 | -1.0743
-0.299   1.371    -2.1     -0.04896 0.04151  0.006815 | -1.0743
iteration 7...
-0.299   1.371    -2.1     -0.04896 0.04151  0.006815 | -1.0743
-0.299   1.371    -2.1     -0.04896 0.04151  0.006815 | -1.0743
-0.299   1.371    -2.112   -0.04896 0.04151  0.006815 | -1.0743
-0.299   1.371    -2.112   -0.04896 0.04151  0.006815 | -1.0743
-0.299   1.371    -2.112   -0.04896 0.04151  0.006815 | -1.0743
-0.299   1.371    -2.112   -0.04896 0.04151  0.006815 | -1.0743
iteration 1...
-0.4476  1.371    -2.112   -0.04896 0.04151  0.006815 | -1.0739
-0.4476  1.426    -2.112   -0.04896 0.04151  0.006815 | -1.0739
-0.4476  1.426    -2.105   -0.04896 0.04151  0.006815 | -1.0739
-0.4476  1.426    -2.105   -0.04706 0.04151  0.006815 | -1.074
-0.4476  1.426    -2.105   -0.04706 0.04307  0.006815 | -1.074
-0.4476  1.426    -2.105   -0.04706 0.04307  0.006815 | -1.074
iteration 2...
-0.4951  1.426    -2.105   -0.04706 0.04307  0.006815 | -1.074
-0.4951  1.365    -2.105   -0.04706 0.04307  0.006815 | -1.074
-0.4951  1.365    -2.096   -0.04706 0.04307  0.006815 | -1.074
-0.4951  1.365    -2.096   -0.04562 0.04307  0.006815 | -1.074
-0.4951  1.365    -2.096   -0.04562 0.04395  0.006815 | -1.074
-0.4951  1.365    -2.096   -0.04562 0.04395  0.007024 | -1.074
-0.5552  1.288    -2.085   -0.04379 0.04506  0.00729  | -1.074
iteration 3...
-0.5648  1.288    -2.085   -0.04379 0.04506  0.00729  | -1.074
-0.5648  1.288    -2.085   -0.04379 0.04506  0.00729  | -1.074
-0.5648  1.288    -2.089   -0.04379 0.04506  0.00729  | -1.074
-0.5648  1.288    -2.089   -0.04419 0.04506  0.00729  | -1.074
-0.5648  1.288    -2.089   -0.04419 0.04506  0.00729  | -1.074
-0.5648  1.288    -2.089   -0.04419 0.04506  0.006512 | -1.074
iteration 4...
-0.5648  1.288    -2.089   -0.04419 0.04506  0.006512 | -1.074
-0.5615  1.292    -2.09    -0.04429 0.045    0.006498 | -1.074
-0.5615  1.292    -2.09    -0.04429 0.045    0.006498 | -1.074
-0.5615  1.292    -2.09    -0.04429 0.045    0.006498 | -1.074
-0.5615  1.292    -2.09    -0.04429 0.045    0.006498 | -1.074
-0.5615  1.292    -2.09    -0.04429 0.045    0.006498 | -1.074
Done    'Coregister: Estimate'
Done
 
Warning: Directory already exists. 
> In DPARSFA_run>(parfor body) at 1595
  In parallel_function at 465
  In DPARSFA_run at 1593
  In DPARSFA>pushbuttonRun_Callback at 1601
  In gui_mainfcn at 96
  In DPARSFA at 33 
Copying coregistered T1 image Files from "T1ImgCoreg":Sub_3047 OK
Segment Setup:Sub_3047 OK
 
 
------------------------------------------------------------------------
Running job #1
------------------------------------------------------------------------
Running 'New Segment'
Done    'New Segment'
Done
 
Running DARTEL: Create Template.
 
 
------------------------------------------------------------------------
Running job #1
------------------------------------------------------------------------
Running 'Run DARTEL (create Templates)'
 0.037776, 0.00107215, 8.57421e-05, 6.33367e-06, 4.67697e-07, 3.82262e-08, 4.00248e-09, 6.80619e-10,
1 1 531473 0 531473 17.867
 0.037032, 0.00106657, 9.29173e-05, 7.74053e-06, 7.14793e-07, 7.76323e-08, 1.08268e-08, 2.08312e-09,
2 1 527671 84.5116 527756 21.6336
 0.0374948, 0.00108128, 9.40618e-05, 7.85521e-06, 7.27386e-07, 7.96331e-08, 1.1356e-08, 2.27752e-09,
3 1 527037 97.6515 527134 18.8079
 0.0372256, 0.00107398, 9.33836e-05, 7.79999e-06, 7.22603e-07, 7.89594e-08, 1.11063e-08, 2.14853e-09,
4 1 527063 27.0924 527091 8.94825
 0.037521, 0.00108949, 9.9877e-05, 8.7542e-06, 8.85049e-07, 1.09369e-07, 1.80438e-08, 4.3216e-09,
5 1 524508 81.4627 524589 11.3327
 0.0373758, 0.00108365, 9.92541e-05, 8.69583e-06, 8.79014e-07, 1.08683e-07, 1.79485e-08, 4.29731e-09,
6 1 524505 57.3829 524562 7.95679
 0.0373852, 0.00108491, 9.93682e-05, 8.70133e-06, 8.78187e-07, 1.08092e-07, 1.76477e-08, 4.12968e-09,
7 1 524504 24.9457 524529 8.29558
 0.0373321, 0.00108083, 0.000102968, 9.23284e-06, 9.69249e-07, 1.25419e-07, 2.17615e-08, 5.47225e-09,
8 1 523126 79.418 523206 18.5078
 0.037419, 0.00108317, 0.000103178, 9.25252e-06, 9.71373e-07, 1.25636e-07, 2.17558e-08, 5.45031e-09,
9 1 523028 83.4892 523111 13.3843
 0.0372958, 0.0010797, 0.000102814, 9.21879e-06, 9.67828e-07, 1.25158e-07, 2.16347e-08, 5.39046e-09,
10 1 523022 27.9982 523050 5.72211
 0.0374001, 0.00108177, 0.000106065, 9.65919e-06, 1.03985e-06, 1.37415e-07, 2.41723e-08, 6.08559e-09,
11 1 522189 71.8894 522261 13.0367
 0.0373242, 0.00107746, 0.000105614, 9.61811e-06, 1.036e-06, 1.37159e-07, 2.42236e-08, 6.13442e-09,
12 1 522197 62.69 522260 15.1689
 0.0373684, 0.00108009, 0.000105881, 9.63889e-06, 1.03692e-06, 1.368e-07, 2.39705e-08, 5.9903e-09,
13 1 522158 31.4346 522190 6.30449
 0.0373149, 0.00107532, 0.000107569, 9.89959e-06, 1.0903e-06, 1.51152e-07, 4.15439e-08, 6.61193e-08,
14 1 521710 64.1202 521774 11.6643
 0.0374183, 0.00107928, 0.000107968, 9.94203e-06, 1.10794e-06, 2.58354e-07, 6.44106e-07, 4.82207e-06,
15 1 521929 62.3328 521991 19.7212
 0.0372923, 0.00107506, 0.000107486, 9.88614e-06, 1.08653e-06, 1.45676e-07, 2.57739e-08, 6.45635e-09,
16 1 521714 173.701 521888 16.8055
 0.037433, 0.00107766, 0.000109363, 1.01261e-05, 1.1337e-06, 1.54559e-07, 2.79263e-08, 8.04418e-09,
17 1 521395 63.277 521458 6.46582
 0.0373504, 0.00107353, 0.000108912, 1.00866e-05, 1.18624e-06, 1.07411e-06, 5.23171e-06, 2.19312e-05,
18 1 522201 40.0974 522241 30.0994
 0.0374565, 0.0010789, 0.000109366, 1.01165e-05, 1.13158e-06, 1.53973e-07, 2.74965e-08, 6.91562e-09,
Done    'Run DARTEL (create Templates)'
Done
 
Running DARTEL: Normalize to MNI space for VBM. Modulated version With smooth kernel [8 8 8].
 
 
------------------------------------------------------------------------
Running job #1
------------------------------------------------------------------------
Running 'Normalise to MNI Space'
 
** "u_rc1BiasField_BiasField_BiasField_co_Mprage_Template" **
c1BiasField_BiasField_BiasField_co_Mprage 1,1,1
c2BiasField_BiasField_BiasField_co_Mprage 1,1,1
c3BiasField_BiasField_BiasField_co_Mprage 1,1,1
Done    'Normalise to MNI Space'
Done
 
Running DARTEL: Normalize to MNI space for VBM. Modulated version.
 
 
------------------------------------------------------------------------
Running job #1
------------------------------------------------------------------------
Running 'Normalise to MNI Space'
 
** "u_rc1BiasField_BiasField_BiasField_co_Mprage_Template" **
c1BiasField_BiasField_BiasField_co_Mprage 1,1,1
c2BiasField_BiasField_BiasField_co_Mprage 1,1,1
c3BiasField_BiasField_BiasField_co_Mprage 1,1,1
Done    'Normalise to MNI Space'
Done
 
Running DARTEL: Normalize to MNI space for VBM. Unmodulated version.
 
 
------------------------------------------------------------------------
Running job #1
------------------------------------------------------------------------
Running 'Normalise to MNI Space'
 
** "u_rc1BiasField_BiasField_BiasField_co_Mprage_Template" **
c1BiasField_BiasField_BiasField_co_Mprage 1,1,1
c2BiasField_BiasField_BiasField_co_Mprage 1,1,1
c3BiasField_BiasField_BiasField_co_Mprage 1,1,1
BiasField_BiasField_BiasField_co_Mprage 1,1,1
Done    'Normalise to MNI Space'
Done
 
Error using DPARSFA_run>(parfor body) (line 2058)
Unable to read file 'F:\wellbeing\test\resttest\RealignParameter\Sub_3047\_Sub_3047.txt': no such file or
directory.
 
Error in DPARSFA_run (line 2012)
        parfor i=1:AutoDataProcessParameter.SubjectNum
 
Error in DPARSFA>pushbuttonRun_Callback (line 1601)
    [Error]=DPARSFA_run(handles.Cfg);
 
Error in gui_mainfcn (line 96)
        feval(varargin{:});
 
Error in DPARSFA (line 33)
    gui_mainfcn(gui_State, varargin{:});
 
Caused by:
    Error using load
    Unable to read file 'F:\wellbeing\test\resttest\RealignParameter\Sub_3047\_Sub_3047.txt': no such file
    or directory.
 
Error while evaluating uicontrol Callback
 
>> 

Hi Xiangrui,

Seems one of your parameters setting is pretty weird.

You set up that you need "Head motion scrubbing regressors", however, seems you didn't set up any parameters in that sub GUI (see the figure below).

Please re-setup your parameters with these parameters correctly checked.

Best,

Chao-Gan

 

Hi Xiangrui,

I hope you are aware that you've checked two scrubbings (see below).

The first one is scrubbing within regression, similar to Satterthwaite et al., 2013 and Yan et al., 2013.

The second one is scrubbing (delete) volumes after regression, similar to Power et al., 2012; 2013.

The GUI you showed is for the second one, but not for the first one.

In generally, you should NOT do scrubbing twice. Please only choose one, and I will suggest the first one.

For some related issues, you can refer to: Yan, C.G., Cheung, B., Kelly, C., Colcombe, S., Craddock, R.C., Di Martino, A., Li, Q., Zuo, X.N., Castellanos, F.X., Milham, M.P., 2013. A comprehensive assessment of regional variation in the impact of head micromovements on functional connectomics. Neuroimage 76, 183-201.

Best,

Chao-Gan

 

Thank you for the prompt detailed reply. Unfortunately, it seems the error is irrevalent to the Scrubbing Setting. After I removed the checkbox for second Scrubbing,  the same error (see following) still occured. Do you have any idea or suggestion to try? Thank you again. -Xiangrui

======================================

Error using DPARSFA_run>(parfor body) (line 2058)

Unable to read file 'F:\wellbeing\test\resttest\RealignParameter\Sub_3047\_Sub_3047.txt': no such file or
directory.
 
Error in DPARSFA_run (line 2012)
        parfor i=1:AutoDataProcessParameter.SubjectNum
 
Error in DPARSFA>pushbuttonRun_Callback (line 1601)
    [Error]=DPARSFA_run(handles.Cfg);
 
Error in gui_mainfcn (line 96)
        feval(varargin{:});
 
Error in DPARSFA (line 33)
    gui_mainfcn(gui_State, varargin{:});
 
Caused by:
    Error using load
    Unable to read file 'F:\wellbeing\test\resttest\RealignParameter\Sub_3047\_Sub_3047.txt': no such file
    or directory.
 
Error while evaluating uicontrol Callback

The error is specifically for the FIRST check box.
Best,

Chao-Gan


On Mon, Sep 30, 2013 at 6:58 PM, The R-fMRI Network <rfmri.org@gmail.com> wrote:
[To post a comment, please reply to rfmri.org@gmail.com ABOVE this line]

Commented by Xiangrui Li (xiangrui)

Thank you for the prompt detailed reply. Unfortunately, it seems the error is irrevalent to the Scrubbing Setting. After I removed the checkbox for second Scrubbing,  the same error (see following) still occured. Do you have any idea or suggestion to try? Thank you again. -Xiangrui

======================================

Error using DPARSFA_run>(parfor body) (line 2058)

Unable to read file 'F:\wellbeing\test\resttest\RealignParameter\Sub_3047\_Sub_3047.txt': no such file or
directory.
 
Error in DPARSFA_run (line 2012)
        parfor i=1:AutoDataProcessParameter.SubjectNum
 
Error in DPARSFA>pushbuttonRun_Callback (line 1601)
    [Error]=DPARSFA_run(handles.Cfg);
 
Error in gui_mainfcn (line 96)
        feval(varargin{:});
 
Error in DPARSFA (line 33)
    gui_mainfcn(gui_State, varargin{:});
 
Caused by:
    Error using load
    Unable to read file 'F:\wellbeing\test\resttest\RealignParameter\Sub_3047\_Sub_3047.txt': no such file
    or directory.
 
Error while evaluating uicontrol Callback

Online version of this post: http://rfmri.org/comment/2893#comment-2893


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Hi there,

I encountered a similar issue and searched online, and the search results lead me to this page of disucssion, wondering if it has been solved? Here is the log in our trial run: 

??? Error using ==> load
Unable to read file C:\Users\Bridget\Documents\MATLAB\DataAnalysis\RealignParameter\Sub_001\: No such file or
directory.
 
Error in ==> DPARSFA_run>(parfor body) at 2482
                Q1=load([AutoDataProcessParameter.DataProcessDir,filesep,'RealignParameter',filesep,AutoDataProcessParameter.SubjectID{i},filesep,DirRP.name]);
                
Error in ==> parallel_function at 469
            F(base, limit);
 
Error in ==> DPARSFA_run at 2453
        parfor i=1:AutoDataProcessParameter.SubjectNum
 
Error in ==> DPARSFA_RerunWithGSR at 87
[Error]=DPARSFA_run(Cfg);
 
Error in ==> DPARSFA>pushbuttonRun_Callback at 1764
        [Error]=DPARSFA_RerunWithGSR(handles.Cfg);
 
Error in ==> gui_mainfcn at 96
        feval(varargin{:});
 
Error in ==> DPARSFA at 30
    gui_mainfcn(gui_State, varargin{:});
 
??? Error while evaluating uicontrol Callback

Nope, but I saw in the online demo that the only two image folders you prepared were FunRaw and T1Raw, so I had the impression that Realignment might happen as a intermediate step before the analysis I choose to do. So I should realign the images as a separate step right?