about proprecessing without smooth

Hi, I'm doing a research about difine several ROIs in a small region, such as PCC, and try to compare the releationship with eachother.

considering  the distance is close, I'm not sure the releationship is true or just because of the smooth to make these neighbor ROIs have similar activity.

do you think it would be help if I skip the step of smooth when I do the proprecessing?

thanks a lot!